Fig. 7: PTTG3P was transcriptionally activated by FoxM1. | Cell Death Discovery

Fig. 7: PTTG3P was transcriptionally activated by FoxM1.

From: A novel lncRNA PTTG3P/miR-132/212-3p/FoxM1 feedback loop facilitates tumorigenesis and metastasis of pancreatic cancer

Fig. 7

a The qRT-PCR analysis revealed upregulation of PTTG3P in the PANC-1 cells transiently transfected with FoxM1(left) and reduction of PTTG3P in the AsPC-1 cells following the depletion of endogenous FOXM1 by siRNA(#1 and #2)(right). b The ability of FoxM1 to bind promoter region of PTTG3P were confirmed in PANC-1 and AsPC-1 cells. c A schematic diagram illustrating the PTTG3P promoter luciferase reporter constructs: TFBS1 with FBS1-FBS3 and TFBS2 only with FBS4. TFBS, transcription binding site. FBS, FoxM1 binding site. d The luciferase promoter assay in 293T cells demonstrated FOXM1 transcriptionally activated PTTG3P by binding to the TFBS1, instead of TFBS2. e The ChIP assay demonstrated that PCR fragments covering TFBS1 were pulled down with the FOXM1 antibody in both PANC-1(left) and AsPC-1 cells (right). f Schematic model shows the results of the study. *P < 0.05, Means ± SD was shown. The experiments were performed in triplicate.

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