Fig. 4: Infusion of MSCs induces erythroid-specific transcription factors and lymphopoiesis.

Splenocytes were harvested at day 7 post-infection from Pb infected mice either infused with or without MSCs isolated from infected mice. RNA was used to perform relative quantification of the erythroid-specific transcription factors GATA-1, GATA-2 and GATA-3 (A). The number of CD4⁺ and CD8⁺ T cells from Pb infected mice either infused with or without MSCs was determined by staining with specific antibodies for cell surface markers and analysed by flow cytometry. B, C Percentage of CD4⁺ T cells and CD8⁺ T cells and D absolute number of CD4⁺ T cells and CD8⁺ T cells (n = 9). Statistical significance was determined by unpaired two-tailed Student’s t-test; **p < 0.01 and ***p < 0.005.