Fig. 1: The hyperglycemic shift to necroptosis depends on mitochondrial ROS.
From: Mitochondrial ROS prime the hyperglycemic shift from apoptosis to necroptosis
U937 cells were grown in 10 or 50 mM glucose followed by the treatment with TNF-α/CHX for 4 h. Cells were then stained with A CellROX Green or B MitoSOX Red, and analyzed by flow cytometry. There is a robust increase in staining for both reagents in cells treated with TNF-α/CHX in 50 mM glucose. U937 cells were grown in 10 or 50 mM glucose followed by the treatment with TNF-α/CHX for 24 h in the presence of vehicle controls or C mitochondrial ROS scavenger, mitoTEMPO, D catalase, and E NADPH oxidase inhibitor, VAS-2870. WST-1 viability assays revealed that cell death in 50 mM glucose was prevented by mitoTEMPO and none of the other inhibitors. All results are from three independent experiments. Graphed values represent mean ± standard deviation. Two-way ANOVA, ***p < 0.001.
