Fig. 5: LINC00958/miR-378a-3p targeted YY1.
From: m6A-induced LINC00958 promotes breast cancer tumorigenesis via the miR-378a-3p/YY1 axis
A Bioinformatics predictive tools (TargetScan, miRDB, PicTar) identified multiple proteins that could be targeted by LINC00958/miR-378a-3p. B Luciferase reporter vectors for YY1 mRNA (wild type and mutant) were synthesized. C Luciferase gene reporter assay found that miR-378a-3p mimics/control connected with the YY1 mRNA wild type/mutant. D RT-PCR analysis found YY1 mRNA in MCF-7 cells transfected with miR-378a-3p mimics/inhibitor or LINC00958 knockdown/overexpression. E T-PCR analysis showed YY1 mRNA expression in MCF-7 cells and normal cells (MCF-10A). F Correlation analysis using Pearson’s χ2 method indicated the correlation with YY1 and LINC00958/miR-378a-3p. **p < 0.01 and *p < 0.05 were considered significant.
