Fig. 2: KDM5A stimulated proliferation in osteosarcoma carcinoma.
From: Histone demethylase KDM5A promotes tumorigenesis of osteosarcoma tumor
A IHC staining of endogenous Ki67 and KDM5A in osteosarcoma and adjacent normal tissues. Adjacent normal tissues and different stages of osteosarcoma tissues (I–II and III stage) were stained with anti-Ki67 (red) and anti-KDM5A (green) antibodies. In the right peak diagram, fluorescent intensity of Ki67 and KDM5A signals across the regions marked with white arrows were shown in line plots, each peak represents the expression of Ki67-positive (Ki67+, red) and KDM5A (KDM5A+, green). Scale bar: 50 μm. B, C Relative Ki67 and KDM5A expression in normal tissues and osteosarcoma tissues of I–II sand III stage were measured by calculating the IntDen/area using ImageJ 1.52a. Expression levels were normalized to DAPI. D Histogram chart showing the percentage of KDM5A+/Ki67+ double-positive cells between adjacent normal and tumor tissues. The differences between groups were assessed using unpaired t test for continuous variables and analysis of variance (ANOVA), *p < 0.05, **p < 0.001.
