Fig. 3: Knockdown of KDM5A reduced osteosarcoma cell proliferation.
From: Histone demethylase KDM5A promotes tumorigenesis of osteosarcoma tumor
A RT-qPCR analysis showed the expression levels of KDM5A in MG-63 and 143B cells with KDM5A knockdown after 48 h (hour). Expression levels were normalized to GAPDH. si-Control, scramble small-interfering RNA; si-KDM5A, small-interfering KDM5A. B Knockdown efficiency of KDM5A in 143B cells was assessed by western blot. GAPDH served as the internal loading control. C The CCK-8 assay was performed to evaluate the proliferation of MG-63 and 143B cells with KDM5A knockdown after 48 h and 72 h. Proliferation rate was normalized to the value at 24 h. D, E The wound-healing assay showed the wound area of MG-63 and 143B cells with KDM5A knockdown after 12 and 24 h. F, G Analysis of the wound-healing area of MG-63 and 143B cells with KDM5A knockdown. The wound-healing area was normalized to the value at 0 h. All data were shown as the mean ± S.D (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001. ns, no significant.
