Fig. 4: ABCA1 expression is correlated with CEBPD expression and promotes against TMZ-induced cell death in GSCs. | Cell Death Discovery

Fig. 4: ABCA1 expression is correlated with CEBPD expression and promotes against TMZ-induced cell death in GSCs.

From: CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 activation for temozolomide resistance in glioblastoma

Fig. 4

A The relative ABCA1 expressions are higher in various grades of human glioma tissues. Expression of ABCA1 was analyzed in tumor tissues of glioma patients compared with normal tissues from the GEO database. B The mRNA expression of ABCA1 positively correlates to CEBPD in glioma samples. C The CEBPD-ABCA1 axis mediates TMZ resistance in GSCs. The knockdown control (siNegative) or CEBPD-knockdown (siCEBPD) U87MG cells were cultured in stem cell medium for 2 days to form spheroids, pretreated with ABCA1 antagonists (400 μM DIDS and 10 μM Probucol) for 24 h, and then treated with 50 μM TMZ for 72 h. The caspase 3/7 activity was detected by the Caspase-Glo® 3/7 Reagent. The summary data are presented as the mean ± SEM; the numbers in the bars represent the sample sizes; one-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001, ns: no significant.

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