Fig. 3: Deficiency of NF90-Ser382 phosphorylation inhibits tumor growth in vivo.
From: NF90 stabilizes cyclin E1 mRNA through phosphorylation of NF90-Ser382 by CDK2
a Cyclin E1 protein levels were detected in xenograft tissues. Three random xenograft tissues per group were selected and homogenized to examine cyclin E1 protein levels by WB. b Mice xenografts of Huh7 cells expressing NF90-WT, NF90-S382, or control. Tumor weight (c) and volume (d) were calculated among NF90-WT, NF90-S382 and control expressed Huh7 cells. c Columns, mean (n = 7); bar, s.d. ***p < 0.001 (d) Points, mean (n = 7); bar, s.d. **p < 0.01; ***p < 0.001. e Ki-67 expression was investigated by IHC analysis, scale bar, 25 μm. f Ki-67 positive cells were assessed in Huh7 cells expressing NF90-WT and NF90-S382. Columns, mean (n = 4); bar, s.d. **p < 0.01. g NF90-S382 phosphorylation positive cells were shown in Huh7 cells expressing NF90-WT and NF90-S382. Columns, mean (n = 4); bar, s.d. **p < 0.01.
