Fig. 4: Expression of BASP1-AS1 and BASP1 during hNPC differentiation. | Cell Death Discovery

Fig. 4: Expression of BASP1-AS1 and BASP1 during hNPC differentiation.

From: Temporal transcriptome analysis of neuronal commitment reveals the preeminent role of the divergent lncRNA biotype and a critical candidate gene during differentiation

Fig. 4

a Relative expression of both BASP1-AS1 and BASP1 in NPCs (maximum) followed by mature neurons and astrocytes (n = 3 biological replicates, mean ± SD). It is the highest in hNPCs, less in neurons, and least in astrocytes. b, c They show the relative expression of BASP1-AS1 and BASP1, respectively during different days of neural and astroglial differentiation from hNPCs. Both the transcripts show a marked rise on day 1 followed by a subsequent fall during neural differentiation. However, during astroglial differentiation, there was a sharp fall on day 1. *p ≤ 0.05, **p ≤ 0.005, and ***p ≤ 0.0005 (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). d, e This figure shows that BASP-AS1 knockdown results in a fall in BASP1 transcript levels in hNPCs. **p ≤ 0.005 (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). f This figure shows that BASP-AS1 knockdown results in a fall in BASP1 transcript levels in hNPCs. **p ≤ 0.005 (n = 3 biological replicates, mean ± SD, Student's two-tailed t test), but no significant effect on BASP1-AS1 transcript on BASP1 knockdown.

Back to article page