Fig. 5: BASP1-AS1 affects neuronal differentiation and neurite length of hNPCs. | Cell Death Discovery

Fig. 5: BASP1-AS1 affects neuronal differentiation and neurite length of hNPCs.

From: Temporal transcriptome analysis of neuronal commitment reveals the preeminent role of the divergent lncRNA biotype and a critical candidate gene during differentiation

Fig. 5

a–f Percentage of DCX + cells decreases significantly after BASP-AS1-KD as compared with scrambled controls on both DAY1 and DAY3 of neural differentiation, respectively. We found that there were approximately fourfold higher DCX + cells in control as compared with BASP1-AS1-KD samples on day 1 of neural differentiation, and approximately twofold higher on day 3. **p ≤ 0.005 and ***p ≤ 0.0005. Scale bar, 50 μm (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). g–i Percentage of Tuj1+ cells decreases significantly after BASP-AS1-KD as compared with scrambled controls on DAY1 of neuronal differentiation. We found that there were approximately twofold higher Tuj1+ cells in control as compared with BASP1-AS1-KD samples on day 1 of neuronal differentiation. *p ≤ 0.05. Scale bar, 50 μm. (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). j–l There is a significant decrease in neurite length on DAY3 of neural differentiation in BASP1-AS1-KD as compared with scrambled controls. As determined by ImageJ with plugin called “Neurite Tracer”. *p ≤ 0.05. Scale bar, 50 μm (n = 3 biological replicates, mean ± SD, Student's two-tailed t test).

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