Fig. 6: BASP1-AS1 affects neuronal proliferation and alters cell-cycle dynamics. | Cell Death Discovery

Fig. 6: BASP1-AS1 affects neuronal proliferation and alters cell-cycle dynamics.

From: Temporal transcriptome analysis of neuronal commitment reveals the preeminent role of the divergent lncRNA biotype and a critical candidate gene during differentiation

Fig. 6

a–f There is an increase in Ki67-positive cells significantly after BASP1-AS1-KD as compared with scrambled controls on both DAY1 and DAY3 of neural differentiation, respectively. *p ≤ 0.05. Scale bar, 50 μm (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). g, h Representative images of DNA content analysis by propidium iodide (PI) staining using FACS 24 h post transfection of either scrambled siRNA or BASP1-AS1 siRNA in human hNPCs. i Bar graph shows quantitative analysis of data obtained from PI staining depicting the percentage of cells in G0/G1, S phase, and G2/M phase of the cell cycle in indicated groups. *p ≤ 0.05 (n = 3 biological replicates, mean ± SD, Student's two-tailed t test).

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