Fig. 8: BASP1-AS1, BASP1, and TCF12 are essential for neural differentiation from hNPCs. | Cell Death Discovery

Fig. 8: BASP1-AS1, BASP1, and TCF12 are essential for neural differentiation from hNPCs.

From: Temporal transcriptome analysis of neuronal commitment reveals the preeminent role of the divergent lncRNA biotype and a critical candidate gene during differentiation

Fig. 8

a, b Percentage of DCX + cells decreases significantly after BASP1-KD as compared with scrambled controls on both DAY1 and DAY3 of neural differentiation, respectively. We found that there were approximately threefold higher DCX + cells in control as compared with BASP1-KD samples on day 1 of neural differentiation and approximately twofold higher on day 3. **p ≤ 0.005 and *p ≤ 0.05 (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). c Percentage of Tuj1+ cells decreases significantly after BASP1-KD as compared with scrambled controls on DAY1 of neuronal differentiation. We found that there were approximately twofold higher Tuj1+ cells in control as compared with BASP1-KD samples on day 1 of neuronal differentiation. *p ≤ 0.05. (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). d, e There is significant increase in Ki67-positive cells after BASP1-KD as compared with scrambled controls on both DAY1 and DAY3 of neural differentiation, respectively. *p ≤ 0.05 (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). f, g Percentage of DCX + cells decreases significantly after TCF12-KD as compared with scrambled controls on both DAY1 and DAY3 of neural differentiation, respectively. *p ≤ 0.05 (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). h Percentage of Tuj1+ cells decreases significantly after TCF12-KD as compared with scrambled controls on DAY1 of neuronal differentiation. **p ≤ 0.005 (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). i, j There is significant increase in Ki67-positive cells after TCF12-KD as compared with scrambled controls on both DAY1 and DAY3 of neural differentiation, respectively. *p ≤ 0.05 (n = 3 biological replicates, mean ± SD, Student's two-tailed t test). k, m Heatmaps showing the effect of BASP1-AS1, BASP1, and TCF12 knockdown, respectively on an array of neurogenesis-related genes. n Venn diagram illustrating the differential expression of overlapping genes in three indicated knockdown conditions. o Bar graph showing that a common set of three genes (NEUROG2, NEUROD1, and HES1) were significantly downregulated in all the indicated three conditions of knockdown (BASP1-AS1, BASP1, and TCF12). *p ≤ 0.05, **p ≤ 0.005. The results are expressed in the ratio of knockdown/scrambled (KD/SCR) (n = 3 biological replicates, mean ± SD, Student's two-tailed t test).

Back to article page