Fig. 2: Tet2 promotes astrocyte differentiation.
From: Tet2-mediated epigenetic drive for astrocyte differentiation from embryonic neural stem cells
a a schema depicting the experimental design. b qRT-PCR of Tet2 demonstrating the extents of overexpression and knockdown of Tet2 (ctrl vs. Tet2OE, p = 0.0073; ctrl vs. Tet2KD, p = 0.0161; n = 12 from three independent biological experiments. Two-tailed Student’s t-test, *p < 0.05, **p < 0.01, Mean ± SD). c Representative Western blot analysis of Gfap protein levels in Tet2KD and Tet2OE NSCs, 7 days after differentiation. The beta-Actin was used as the internal control. d Quantitative PCR analysis of lineage markers Gfap, Tubb3, mRNA levels in control (scrambled), Tet2 overexpressed-NSCs (Tet2OE) and Tet2 knocked-down NSCs (Tet2KD), 7 days after differentiation. (Gfap, ctrl vs. Tet2OE, p = 0.041; ctrl vs. Tet2KD, p = 0.0007; Tet2KD vs. Tet2OE, p = 0.0001; n = 15, from three independent biological repeats. One-way ANOVA, Tukey test, *p < 0.05, ***p < 0.001, ns, nonsignificant, Mean ± SD).
