Fig. 3: Olig2 binds to Tet2 promoter and represses the expression of Tet2.

a ChIP-chip and ChIP-qPCR validation of Olig2 targets (Tet2, Nfia) and non-Olig2 targets (Gfap, S100b) in E11 cortical NSCs (Tet2 vs. Gfap p = 0.012, n = 15 from three independent biological repeats. Two-tailed Student’s t-test, *p < 0.05, ns nonsignificant, Mean ± SD). ChIP-chip replicates are shown in red and blue bars, the height of the bar plot indicates the enrichment of Olig2 at the corresponding locus, black arrow indicates the direction of transcription, blue bars at the bottom of the graphs indicates the true binding peaks identified by MACS. b Enrichment of RNA polymerase II, Olig2 at Tet2 and Gfap TSS during NSC spontaneous differentiation (IP Olig2, Tet2TSS, Day0 vs. Day7 p = 0.032. IP PolII, Gfap TSS, Day0 vs. Day7 p = 0.011, n = 6 from two independent biological repeats. Nonparametric t-test, *p < 0.05, ns nonsignificant, Mean ± SD). c Overexpression of Olig2 suppressed Tet2 gene expression (p = 0.0076, n = 15 from three independent biological repeats. Two-tailed Student’s t-test, **p < 0.01, Mean ± SD). d The expression of Olig2 anti-correlates with the expression of Tet2 and Gfap during astrogliogenesis (Olig2, p = 0.0024; Tet2: p = 0.0095; Gfap, p = 0.013. n = 12 from three independent biological repeats. Two-tailed Student’s t-test, *p < 0.05, **p < 0.01, Mean ± SD).