Fig. 4: Olig2 and Tet2 regulated astrogliogenesis in an opposite manner.
From: Tet2-mediated epigenetic drive for astrocyte differentiation from embryonic neural stem cells
a Overexpression of Olig2 regulated neural lineage genes expression (Cnp, p = 0.0018; Gfap, p = 0.013; Tubb3, p = 0.031; Id2, p = 0.0035; n = 15 from three independent biological repeats. Two-tailed Student’s t-test, *p < 0.05, **p < 0.01, Mean ± SD). b, c Immunohistochemistry and quantification of Gfap+ astrocytes, Map2+ neurons, Cnp+ oligodendrocytes in ctrl and Tet2 or Olig2 overexpressed E11 NSCs (Gfap,ctrl vs. Tet2OE, p = 0.0091; ctrl vs Olig2OE, p = 0.0153; Tet2OE vs. Olig2OE, p = 0.0011; Map2, ctrl vs. Olig2OE, p = 0.0209; Tet2OE vs. Olig2OE, p = 0.0446, n = 18, three independent biological repeats. One-way ANOVA, Tukey test, *p < 0.05, ns nonsignificant, Mean ± SD). Scale bars, 50 μm.
