Fig. 5: ST2 neutralization antibody relieved the disease severity of BPD. | Cell Death Discovery

Fig. 5: ST2 neutralization antibody relieved the disease severity of BPD.

From: IL-33-induced neutrophil extracellular traps degrade fibronectin in a murine model of bronchopulmonary dysplasia

Fig. 5

E14–15d pregnant dams were intraperitoneally injected with 75 µg/kg LPS, and newborn mice were exposed to 65% hyper-oxygen to establish BPD model. On the 7th and 10th day after birth, isotype control antibody or ST2-neutralizing antibody was simultaneously intraperitoneally injected into the pups, and the pups were sacrificed at day 14 post birth. a Design test plots for the use of isotype controls and ST2-neutralizing antibodies. b–e Lung morphometry was analyzed by H&E staining of tissue samples from surviving pups at day 14 post birth. Scale bars = 200 μm. f Fibronectin protein expression in pulmonary tissues at day 14 post birth. g Representative results of immunohistochemical analysis of fibronectin in pulmonary tissues at day 14 post birth. Three mice were included in the control and BPD group at each time point. All experiments were repeated at least three times. Scale bars, 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001.

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