Fig. 4: KCNA1 and GPC1 were validated to be direct targets of miR-125b. | Cell Death Discovery

Fig. 4: KCNA1 and GPC1 were validated to be direct targets of miR-125b.

From: Elevated microRNA-125b inhibits cytotrophoblast invasion and impairs endothelial cell function in preeclampsia

Fig. 4

The human trophoblast cells, HTR8/SVneo, were transfected with miR-125b mimics or negative control miRs. mRNA levels (a) and protein levels (c) of putative target KCNA1 was evaluated post transfection using real-time qPCR and western blot separately. Data are presented as mean ± SEM of four independent experiments in triplicate. *p < 0.05, **p < 0.01. The human endothelial cells, HUVECs, were transfected with miR-125b mimics or negative control miRs. In total, 48 h later, mRNA levels (b) and protein levels (d) of putative target KCNA1 was evaluated using real-time qPCR and western blot separately. Data are presented as mean ± SEM, N = 4 performed in triplicate. *p < 0.05, **p < 0.01. e, f HEK-293T cells were co-transfected with luciferase reporter construct and miR-125b mimics. Luciferase activities were determined 24 h post transfection. Data are presented as mean ± SEM values of four independent experiments, each conducted in triplicate. *p < 0.05.

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