Fig. 6: miR-125b impaired trophoblast invasion through GPC1. | Cell Death Discovery

Fig. 6: miR-125b impaired trophoblast invasion through GPC1.

From: Elevated microRNA-125b inhibits cytotrophoblast invasion and impairs endothelial cell function in preeclampsia

Fig. 6

HUVECs were transfected with transfected with miR-125b, miR-125b inhibitor, or GPC1 siRNA, and then cultured in the Matrigel-coated plates (BD Bioscience). At 6 h after seeding, images were collected from five random fildes per well using light microscopy. The total tube length was measured and analyzed using Image J, as shown the typical field in a and tube length in b. N = 4 conducted in triplicates and results are presented as mean ± SEM, with p < 0.05 considered as significant. *p < 0.05, **p < 0.01. c Typical microscope fields of tube formation assays in HUVECs transfected with either GPC1 overexpressing pcDNA 4 vector (pcGPC1) or pcDNA 4 vector (pDNA4). miR-125b mimic or negative control was co-transfected. d Tube length were based on four independent experiments carried out in triplicates and results are presented as mean ± SEM. with p < 0.05 considered as significant. *p < 0.05, **p < 0.01.

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