Fig. 1: Upregulation of miR-674-5p in mouse IECs during endotoxemia-induced intestinal injury. | Cell Death Discovery

Fig. 1: Upregulation of miR-674-5p in mouse IECs during endotoxemia-induced intestinal injury.

From: MicroRNA-674-5p induced by HIF-1α targets XBP-1 in intestinal epithelial cell injury during endotoxemia

Fig. 1

a Significant changes in miRNA expression in IECs of small intestines isolated from mice at day 3 after treated with lipopolysaccharide (LPS) or phosphate-buffered saline (PBS) during endotoxemia-induced intestinal injury. Values are presented as means ± standard deviation (SD), n = 6 in each group. *P < 0.05 versus PBS. b Real-time PCR of miR-674-5p. RNA from IECs isolated from small intestines of mice at day 3 after injected with LPS or PBS. Values are presented as means ± SD, n = 6 in each group. *P < 0.01 versus PBS. c Real-time PCR of miR-674-5p. RNA from IECs isolated from small intestines of mice at day 3 after treated with Staphylococcus aureus or PBS. Values are presented as means ± SD, n = 6 in each group. *P < 0.05 versus PBS. d Northern blot analysis of miR-674-5p. Total RNA (10 μg) extracted from IECs isolated from mice at day 3 after treated with LPS, S. aureus, or PBS were used for northern blotting. 5S rRNA was probed as a loading control.

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