Fig. 2: HIF-1α mediates induction of miR-674-5p during LPS stimulation. | Cell Death Discovery

Fig. 2: HIF-1α mediates induction of miR-674-5p during LPS stimulation.

From: MicroRNA-674-5p induced by HIF-1α targets XBP-1 in intestinal epithelial cell injury during endotoxemia

Fig. 2

a Induction of miR-674-5p by LPS stimulation. IEC-6 cells were stimulated for 12–48 h to extract RNA for real-time PCR analysis of miR-674-5p. Fold changes over the value of IECs treated with PBS (arbitrarily set as 1) are shown. Values are presented as means ± SD, n = 6 in each group. *P < 0.01 versus IECs treated with PBS. b Induction of HIF-1α by LPS stimulation. IEC-6 cells were stimulated for 12–48 h to extract RNA for real-time PCR analysis of HIF-1α. Fold changes over the value of IEC cells treated with PBS (arbitrarily set as 1). Values are presented as means ± SD, n = 6 in each group. *P < 0.05 versus IECs with PBS. c Western blot analysis of HIF-1α in IEC-6 cells was carried out with whole cell lysates collected at various time points after LPS stimulation. d IEC-6 cells transfected with a sequence-scrambled oligonucleotide control or HIF-1α small interfering RNA were subjected to LPS stimulation, and whole cell lysates were collected at 24 h. e Induction of miR-674-5p by LPS stimulation. HIF-1α+/+ and HIF-1α–/– mouse embryonic fibroblasts were stimulated for 24 h to extract RNA for real-time PCR analysis of miR-674-5p. Fold changes over the value of HIF-1α+/+ cells with LPS (arbitrarily set as 1) are shown. *P < 0.01 versus HIF-1α+/+ cells with LPS. f HIF-1α binding to the miR-674-5p promoter during LPS stimulation. HIF-1α+/+ and HIF-1α–/– cells were stimulated with LPS or PBS for 24 h. Cell lysates were collected for chromatin immunoprecipitation analysis of HIF-1α binding to miR-674-5p promoter DNA.

Back to article page