Fig. 7: Blockade of miR-674-5p encourages intestinal crypt cell proliferation via XBP-1 under LPS stimulation. | Cell Death Discovery

Fig. 7: Blockade of miR-674-5p encourages intestinal crypt cell proliferation via XBP-1 under LPS stimulation.

From: MicroRNA-674-5p induced by HIF-1α targets XBP-1 in intestinal epithelial cell injury during endotoxemia

Fig. 7

a BrdU staining of intestinal crypt cells isolated from mice at 24 h after LPS stimulation with or without treatment with anti-miR-674 oligonucleotide or the sequence-scrambled oligonucleotide control. b Proliferative index was measured at 24 h after LPS treatment by counting a minimum of 20 randomly selected microscopy fields (×200 magnification) following BrdU staining. The index was obtained by dividing the number of BrdU-positive cells by the total number of cells. *P < 0.01 versus scrambled control. c RT-PCR analysis of Notch1 mRNA in intestinal crypt cells isolated from mice at 24 h after LPS treatment with or without treatment with anti-miR-674 oligonucleotide or the scrambled control. *P < 0.05 versus scrambled control. d RT-PCR analysis of Bmi1 mRNA in intestinal crypt cells isolated from mice at 24 h after LPS treatment with or without treatment with anti-miR-674 or the scrambled. *P < 0.01 versus scrambled control. e Western blot analysis of ER stress-related proteins of intestinal crypt cells at 24 h after LPS stimulation with or without treatment with anti-miR-674 oligonucleotide or the scrambled control. β-actin was used as a loading control.

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