Fig. 4: Biolayer-interferometry for measuring bi-molecular interactions.
From: Biochemical activity of RAGs is impeded by Dolutegravir, an HIV integrase inhibitor
a, b Biolayer interferometry for RAG1 central domain (RAG1-CD) (b) and RAG1 nonamer binding domain (RAG1-NBD) (a). Purified CD or NBD was incubated with increasing concentrations of Dolutegravir (3.125, 6.25, 12.5, 25, 50 and 100 µM) and was evaluated using BLI. BLI analysis showed that RAG1-CD and NBD bound with a Kd of 1.5 nM and 4.6 nM, respectively to biotinylated 12 RSS. But in the presence of Dolutegravir a marginal increase in the binding constant was observed; Kd 4.6 nM for RAG1-CD and Kd 9.3 nM for RAG1-NBD.
