Fig. 4: circPKNOX1 regulated NP cells via miR-370-3p.
From: Inhibition of intervertebral disc disease progression via the circPKNOX1–miR-370-3p–KIAA0355 axis
A Expression of related proteins (collagen II, aggrecan, SOX9, MMP3, MMP13, ADAMTS4, and ADAMTS-5) was reversed. B The fluorescence intensity of related proteins (collagen II, aggrecan, ADAMTS4, and MMP13) was observed via an immunofluorescence assay (scale bar, 100μm). C RT-qPCR was used to detect the expression of related genes (collagen II, aggrecan, SOX9, MMP3, MMP13, ADAMTS4, and ADAMTS5); *P<0.05, **P<0.01. Data represent mean±SD, and the P values were determined by a two-tailed unpaired Student’s t test. D Left: the extracellular matrix was detected by Alcian staining. Right: the Alcian staining was quantitatively analyzed. Data represent mean±SD, and the P values were determined by a two-tailed unpaired Student’s t test.
