Fig. 6: In the animal model, the circPKNOX1/miR-370-3p/ KIAA0355 axis promoted IVDD development.
From: Inhibition of intervertebral disc disease progression via the circPKNOX1–miR-370-3p–KIAA0355 axis
A Left: the tails of the mice were examined using X-ray imaging analysis. Right: the disc height index of the tails of the mice was quantitatively analyzed; *P<0.05. Data represent mean±SD, and the P values were determined by a two-tailed unpaired Student’s t test. B The expression of circPKNOX1 was detected using RT-qPCR; ***P<0.001. Data represent mean±SD, and the P values were determined by a two-tailed unpaired Student’s t test. C The expression of KIAA0355 was detected using RT-qPCR; ***P<0.001. Data represent mean±SD, and the P values were determined by a two-tailed unpaired Student’s t test. D The intervertebral disc tissue of mice was observed using hematoxylin and eosin, Safranin-O/fast green, and toluidine blue staining (scale bar, 200μm). E In the control, IVDD, circPKNOX1-mut, and circPKNOX1-wt groups, the expression of related proteins (collagen II, aggrecan, SOX9, MMP3, MMP13, ADAMTS4, and ADAMTS-5) was detected using western blotting. F Sections of the discs were analyzed using immunohistochemical staining for collagen II, aggrecan, ADAMTS4, and MMP13 (scale bar, 200μm). G The mechanism of action of circPKNOX1.
