Fig. 1: JWA negatively regulates HER2 expression and cell proliferation in breast cancer.
From: JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling
a The mRNA expressions of JWA in breast cancer tissues and adjacent non-cancerous tissues in unpaired cohorts (TCGA database, 1099 cancer samples, 119 non-cancerous samples). b, c Kaplan–Meier curves depicting OS according to the expression patterns of JWA (b) and HER2 (c) in the breast cancer cohort. P values were calculated with the log-rank test. d Kaplan–Meier OS analysis for breast cancer from GEO datasets (GSE88770 and GES42568). e Representative images of JWA IHC staining in breast cancer lesions with absent (−), weak (+), moderate (++), strong (+++) expression of HER2 (original magnification, ×2 and ×200). f Transfection of either Flag-JWA plasmid or si-JWA and their control into BT474 cells for 72 h, the expressions of HER2 and JWA proteins were determined by western blot. g Representative images of the colony formation assay for BT474 after transfection of Flag-JWA, si-JWA or their control. h Immunofluorescence imaging of JWA (green), HER2 (red), nucleus labeled as DAPI (blue), the co-localization of the three signals (merge) in BT474 cells transfected with si-JWA (right) or Flag-JWA for 72 h.
