Fig. 5: JAC1 modulates SMURF1 through JWA-p38-GATA-1-NEDD4 axis.
From: JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling
a, b The endogenous protein–protein interaction between NEDD4 and SMURF1 was identified by IP and western blot in both BT474 and SKBR3 cells. c Western blot analysis of NEDD4 and SMURF1 of SKBR3 cells transfected with si-NEDD4 or si-SMURF1. d The mRNA expressions of NEDD4 and JWA were detected by real-time PCR in SKBR3. The values were calculated as 2−ddCT, and the relative fold change was compared to the control groups after being normalized to GAPDH. e The mRNA expressions of GATA-1, STAT4, STAT1-beta, GR-alpha, and TFIID were detected by real-time PCR in SKBR3. The values were calculated as 2−ddCT, and the relative fold change was compared to the control groups after being normalized to GAPDH. f The regulations of JAC1 on GATA-1 were determined in both BT474 and SKBR3 cells. g JAC1-induced activation of p-p38 was determined by western blot in both BT474 and SKBR3 cells. h Western blot analysis of p-p38, GATA-1, NEDD4, SMURF1, and HER2 of SKBR3 cells treated with JAC1 or SB203580. i The inhibition of JAC1 on colony formation via the p38 MAPK pathway was determined in SKBR3 cells.
