Fig. 5: Knockdown of STAT3 attenuates VNS’s effects on STAT3, Caspase-1, and M1M2 macrophage phenotypes. | Cell Death Discovery

Fig. 5: Knockdown of STAT3 attenuates VNS’s effects on STAT3, Caspase-1, and M1M2 macrophage phenotypes.

From: Vagus nerve stimulation enhances the cholinergic anti-inflammatory pathway to reduce lung injury in acute respiratory distress syndrome via STAT3

Fig. 5

a Fluorescence images of GFP (green) in injected lung 7 days after lentiviruses were stereotaxically injected (scale bar = 100 μm). b Representative western blotting images of STAT3 expression in the LV-con-RNAi+ARDS group, LV-con-RNAi+ARDS+VNS group, LV-STAT3-RNAi+ARDS group, and LV-STAT3-RNAi+ARDS+VNS group (n = 6 biological replicates). c Representative western blotting analysis images of Caspase-1 expression in the LV-con-RNAi+ARDS group, LV-con-RNAi+ARDS+VNS group, LV- STAT3-RNAi+ARDS group, and LV-STAT3-RNAi+ARDS+VNS groups (n = 6 biological replicates). d Comparison of mean intensity ratios for STAT3. e Comparison of mean intensity ratios for Caspase-1. f–i The mRNA levels of the M1 macrophage markers, iNOS and CXCL-9, and those of the M2 macrophage markers, Arg1 and Ym1, were measured in the LV-con-RNAi+ARDS group, LV-con-RNAi+ARDS+VNS group, LV-STAT3-RNAi+ARDS group, and LV-STAT3-RNAi+ARDS+VNS group by qPCR and normalized to GAPDH mRNA levels (n = 6 biological replicates). The results are expressed as the mean ± SEM (n = 6 biological replicates); *p < 0.05, **p < 0.01.

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