Fig. 1: TAC and Ang-II, respectively, induce pyroptosis in myocardial tissue and cultured cardiomyocytes. | Cell Death Discovery

Fig. 1: TAC and Ang-II, respectively, induce pyroptosis in myocardial tissue and cultured cardiomyocytes.

From: NLRP3-mediated pyroptosis aggravates pressure overload-induced cardiac hypertrophy, fibrosis, and dysfunction in mice: cardioprotective role of irisin

Fig. 1

A1 Representative images showing TUNEL + pyroptotic cardiomyocytes. Red—anti-tropomyosin, blue nuclei—DAPI, green nuclei—TUNEL. Scale bars: 100 μm. A2 Percentage of TUNEL-positive cells in the indicated groups. B Immunoblots showing the levels of cleaved caspase-1 (B1, B2), IL-1β (B1, B3), and GSDMD-N (B1, B4) in the indicated groups. C1 Representative images showing PI-stained pyroptotic cardiomyocytes with/out Ang-II treatment. Blue nuclei—DAPI, red nuclei—PI. Scale bars: 200 μm. C2 Percentage of PI-positive cells in the indicated groups. D Immunoblots showing levels of cleaved caspase-1 (D1, D2), IL-1β (D1, D3), and GSDMD-N (D1, D4) in the suitably treated cardiomyocytes. Results are mean ± SEM, n = 6. *P < 0.01 compared to control.

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