Fig. 5: Hif2a deficiency in renal interstitial cells attenuates fibrogenesis induced by UUO injury. | Cell Death Discovery

Fig. 5: Hif2a deficiency in renal interstitial cells attenuates fibrogenesis induced by UUO injury.

From: SIRT1 attenuates renal fibrosis by repressing HIF-2α

Fig. 5

a Genotyping for Hif2a CKO mice. The length of PCR products for the wild-type Hif2a allele is 1834 bp, and the length for the knockout Hif2a allele is 323 bp. b HIF-2α expression was reduced in kidneys from Hif2a CKO mice. Gene expression was analyzed by qRT-PCR and 18S has used a house-keeping gene. c Renal fibrosis was attenuated by Hif2a deficiency. Protein levels were analyzed by western blot and GAPDH was used as a loading control. d Quantitative analysis of western blot data as shown in c. n = 6. Data are means ± SEM. ns: no significance. *P > 0.05, **P < 0.01, ***P < 0.001.

Back to article page