Fig. 5: Crystal internalization induces LDH release and apoptosis in HK2 cells. | Cell Death Discovery

Fig. 5: Crystal internalization induces LDH release and apoptosis in HK2 cells.

From: Correction: Abrogation of store-operated Ca2+ entry protects against crystal-induced ER stress in human proximal tubular cells

Fig. 5

Control (noncrystal) CaP, CaOx, and CaP + CaOx (mixed) crystals were introduced into HK2 cells for 24 h. Cell death was determined by A DAPI staining and B Annexin V labeling. C Cell viability was determined relative to control (100%) by methylene blue staining. D LDH release was determined by measuring conversion of purple tetrazolium salt into red formazan. E Necrosis was detected with PI staining. Statistically significant differences are indicated (mean ± SEM). Experiments were performed in triplicates. Data are presented from three individual experiments (n = 3). Two-tailed t test was used for statistical comparison. Levels of significance are indicated as *p < 0.05 and **p < 0.01 as shown in the bar diagrams. Scale bar, 100 µm.

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