Fig. 2: CircHIPK3 promotes H/R-induced cardiomyocyte apoptosis.

A The intracellular ROS level was detected by flow cytometry. n = 3. B Annexin V-FITC/PI flow cytometry was used to evaluate the effect of circHIPK3 on cardiomyocyte apoptosis. n = 3. C Apoptosis-related proteins, including procaspase-3, cleaved caspase-3, Bax, and Bcl-2, were detected by western blotting. n = 3. *P < 0.05 compared with the normal group. #P < 0.05 compared with the H/R group.