Fig. 3: CircHIPK3 acts as a miR-20b-5p sponge. | Cell Death Discovery

Fig. 3: CircHIPK3 acts as a miR-20b-5p sponge.

From: CircHIPK3 regulates the autophagy and apoptosis of hypoxia/reoxygenation-stimulated cardiomyocytes via the miR-20b-5p/ATG7 axis

Fig. 3

A The localization of circHIPK3 was observed in cardiomyocytes by RNA FISH with a Cy3-labeled circHIPK3 probe. The nuclei were stained with DAPI. Scale bar = 20 μm. B The expression of miR-20b-5p in normal and H/R cardiomyocytes was detected by qRT-PCR. *P < 0.05 compared with the normal group. n = 9. C qRT-PCR analysis of miR-20b-5p expression in cardiomyocytes after cardiomyocytes were transfected with negative control (LV), circHIPK3 overexpression vector, or circHIPK3 siRNA (si-circHIPK3). D The relative luciferase activities were measured in 293T cells cotransfected with circHIPK3-WT or circHIPK3-Mut and miR-20b-5p mimics or miR-NC(MNC) by luciferase reporter assay. *P < 0.05 compared with the circHIPK3-Mut group. E FISH for circHIPK3 (red) and miR-20b-5p (green) was performed in cardiomyocytes. Scale bar = 20 μm.

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