Fig. 4: MiR-20b-5p inhibits autophagy and apoptosis of cardiomyocytes under H/R conditions. | Cell Death Discovery

Fig. 4: MiR-20b-5p inhibits autophagy and apoptosis of cardiomyocytes under H/R conditions.

From: CircHIPK3 regulates the autophagy and apoptosis of hypoxia/reoxygenation-stimulated cardiomyocytes via the miR-20b-5p/ATG7 axis

Fig. 4

A Transfection efficacy of miR-20b-5p mimics and miR-20b-5p inhibitors in cardiomyocytes. B Western blot showed the effect of transfection of miR-20b-5p mimics and miR-20b-5p inhibitors on the expression of LC3II and P62 in normal cardiomyocytes. n = 3. C Western blot showed the effect of transfection of miR-20b-5p mimics and miR-20b-5p inhibitors on the expression of apoptosis-related proteins, including procaspase-3, cleaved caspase-3, Bax, and Bcl-2 in normal cardiomyocytes. n = 3. D Western blot showed the effects of miR-20b-5p mimics and miR-20b-5p inhibitor transfection on the expression of LC3II and P62 in cardiomyocytes under H/R conditions. n = 3. E Annexin V-FITC/PI flow cytometry was used to evaluate the effect of miR-20b-5p mimics and miR-20b-5p inhibitors on cardiomyocyte apoptosis under H/R conditions. n = 3. F Western blot analyzed the expression of apoptosis-related proteins, including procaspase-3, cleaved caspase-3, Bax, and Bcl-2 in cardiomyocytes under H/R conditions by miR-20b-5p mimics and miR-20b-5p inhibitors transfection. n = 3. *P < 0.05 compared with the mimics-NC (MNC) group. #P < 0.05 compared with the inhibitors-NC (INC) group.

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