Fig. 5: CircHIPK3 regulates autophagy and apoptosis via the CircHIPK3/miR-20b-5p/ATG7 axis. | Cell Death Discovery

Fig. 5: CircHIPK3 regulates autophagy and apoptosis via the CircHIPK3/miR-20b-5p/ATG7 axis.

From: CircHIPK3 regulates the autophagy and apoptosis of hypoxia/reoxygenation-stimulated cardiomyocytes via the miR-20b-5p/ATG7 axis

Fig. 5

A Luciferase reporter assay showed that miR-20b-5p mimics directly binds to the 3′-UTR of ATG7 and inhibits luciferase activity. *P < 0.05 compared with the NC-mimics group. B ATG7 protein expression was detected by western blotting. n = 3. *P < 0.05 compared with the MNC group. #P < 0.05 compared with the INC group. C The protein expression levels of LC3-II, P62, and ATG7 were measured by western blotting. n = 3. D The intracellular ROS level was detected by flow cytometry. n = 3. E Annexin V-FITC/PI flow cytometry was used to evaluate apoptosis under different treatment conditions. n = 3. F Apoptosis-related proteins, including procaspase-3, cleaved caspase-3, Bax, and Bcl-2, were detected by western blotting. n = 3. *P < 0.05 compared with the H/R group. #P < 0.05 compared with the H/R + sicircHIPK3 group.

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