Fig. 1: Inactivation of both p21 and ASK1 has not a major impact on differentiation of keratinocytes. | Cell Death Discovery

Fig. 1: Inactivation of both p21 and ASK1 has not a major impact on differentiation of keratinocytes.

From: Functional cooperation between ASK1 and p21Waf1/Cip1 in the balance of cell-cycle arrest, cell death and tumorigenesis of stressed keratinocytes

Fig. 1

A Primary mouse keratinocytes were established from 2-day-old newborn mice and maintained in low calcium medium. Cells were analyzed for western blot analysis with the indicated antibodies 5 days post-seeding. B H&Es taining of single p21KO, ASK1KO, ASK1/p21KO, and wild-type (WT) skin at birth (2-day-old newborn mice). 10X microscope field (scale bar: 100 μm). C Equal amounts of protein extract from WT, p21KO, ASK1KO ASK1/p21KO neonate dorsal skin (2-day-old newborn mice) were separated by SDS-PAGE and analyzed by western blot with antibodies directed against keratin 1 and loricrin. Vinculin has been used as a loading control (D) Immunohistochemical labeling of dorsal skin sections from 2-day-old newborn WT, p21KO, ASK1KO ASK1/p21KO mice using antibodies specific to loricrin shows a similar expression level and localization of the differentiation marker in WT and KO mice. 20X microscope field (scale bar: 100 μm).

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