Fig. 1: APG-115 potently inhibits cellular proliferation and induces apoptosis, with G₀/G₁ cell cycle arrest, in TP53^wt AML cell lines.

A Mean (SD) cellular viability of MOLM-13 (TP53^wt), MV-4-11 (TP53^wt), OCI-AML-3 (TP53^wt), HL-60 (TP53^null), and SKM-1 (TP53^mut) cells treated with increasing concentrations of APG-115 for 72 h (n = 3 replicates). B Total protein levels of MDM2, P53, P21, and the loading control β-actin by Western blot in MOLM-13 cells exposed to APG-115 at increasing concentrations for 4 h. C Representative flow cytometry results from two independent experiments (left panel) and mean (SD) percentage of MOLM-13 cells in apoptosis (right panel) presented as n = 4 technical replicates. *p < 0.0001; ns not significant; compared to dimethyl sulfoxide vehicle (DMSO) via two-tailed Student t tests for data from three independently repeated experiments. D Increased cell cycle arrest in the G₀/G₁ phase, as indicated by flow cytometry in AML cells exposed to increasing concentrations of APG-115 for 48 h. Data shown are representative images of two independent experiments.