Fig. 1: CircRNA profiling in MI hearts. | Cell Death Discovery

Fig. 1: CircRNA profiling in MI hearts.

From: Overexpression of circRNA SNRK targets miR-103-3p to reduce apoptosis and promote cardiac repair through GSK3β/β-catenin pathway in rats with myocardial infarction

Fig. 1

A Heat map of circRNAs differentially expressed at 3 days post MI. [C1, C2, C3, and C4] denotes expression in sham animals while [M1, M2, M3, and M4] denotes animals subject to MI. Relative expression values are indicated on the color key and increase in value from navy to firebrick (n = 4). B Quantitative real-time PCR (qRT-PCR) analysis of differentially expressed circRNAs (n = 3). C Divergent primers amplify circSNRK in cDNA but not genomic DNA (gDNA); divergent and convergent primers are indicated by the direction of the arrow. D The abundances of circSNRK and GAPDH mRNA in cardiomyocytes treated with RNase R, which were normalized to the amount measured in the mock treatment. (n = 3). E qRT-PCR analysis of circSNRK expression at different time points in post MI heart tissues (n = 5) compared to sham (n = 5). F, G qRT-PCR analysis of circSNRK in isolated cardiomyocytes and fibroblasts from neonatal rat hearts under normal and H/SD conditions. (n = 6). H qRT-PCR for the abundance of circSNRK in either the cytoplasm or nucleus of primary CMs. *<0.05, **P < 0.01, ***p < 0.001.

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