Fig. 6: Overexpression of circSNRK reduces cardiomyocyte apoptosis and promotes adult cardiac regeneration after MI. | Cell Death Discovery

Fig. 6: Overexpression of circSNRK reduces cardiomyocyte apoptosis and promotes adult cardiac regeneration after MI.

From: Overexpression of circRNA SNRK targets miR-103-3p to reduce apoptosis and promote cardiac repair through GSK3β/β-catenin pathway in rats with myocardial infarction

Fig. 6

A TUNEL immunofluorescence staining in the AAV9 ctrl and AAV9 circSNRK groups 7 days post MI and quantification of TUNEL positive CMs. ***p < 0.001 vs. Sham + AAV9 ctrl, ###p < 0.001 vs. MI + AAV9 ctrl; bar = 25 µm. (n = 5–6 for each group). B BCL-2, BAX, cleaved-caspase-3/caspase-3 levels in heart tissue after AAV9 ctrl and AAV9 circSNRK treatment. ***p < 0.001 vs. Sham + AAV9 ctrl, ###p < 0.001 vs. MI + AAV9 ctrl (n = 3). C Ki67 immunofluorescence staining in adult rat hearts 14 days after transfection with AAV9 circSNRK and AAV9 ctrl and quantification of Ki67-positive cardiomyocytes. ***p < 0.001 vs. Sham + AAV9 ctrl group; ###p < 0.001 vs. MI + AAV9 ctrl group. bar = 25 µm (n = 5–6 for each group). D α-SMA (α-smooth muscle actin) staining in the AAV9 ctrl and AAV9 circSNRK groups 14 days after transfection with AAV9 circSNRK and AAV9 ctrl and quantification of α-SMA-positive capillaries, ***p < 0.001 vs. Sham + AAV9 ctrl group; ###p < 0.001 vs. MI + AAV9 ctrl group. bar = 25 µm (n = 5–6 for each group). E CD31 staining in the border zone of ischemic hearts and quantification of CD31 positive blood vessels. **p < 0.01 vs. Sham + AAV9 ctrl group; ###p < 0.001 vs. MI + AAV9 ctrl group. bar = 25 µm (n = 5–6 for each group).

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