Fig. 7: SNRK protects cardiomyocytes through GSK3β/β-catenin pathway. | Cell Death Discovery

Fig. 7: SNRK protects cardiomyocytes through GSK3β/β-catenin pathway.

From: Overexpression of circRNA SNRK targets miR-103-3p to reduce apoptosis and promote cardiac repair through GSK3β/β-catenin pathway in rats with myocardial infarction

Fig. 7

A Representative Western blot and quantified data show expression of GSK3β, phosphorylation of GSK3β, and β-catenin in hypoxic and ischemic NRCMs treated with SNRK overexpression ***p < 0.001 vs. ctrl + SNRK-NC group; ###p < 0.001 vs. H/SD + SNRK-NC group. (n = 3). B NRCMs were pretreated with DMSO or a GSK3β inhibitor for 2 h, followed by incubation with in hypoxic and ischemic condition for 12 h. Representative western blot and quantified data show expression of GSK3β, p-GSK3β, and β-catenin. ***p < 0.001 relative to DMSO in each group (n = 3). C Representative western blot and quantified data show expression of BCL-2, BAX, cleaved-caspase-3/caspase3. ***p < 0.001 relative to DMSO in each group (n = 3). D The interaction between SNRK and GSK3β in cardiomyocytes was assessed using immunoprecipitation and western blot analysis. Input, total cell lysates; IgG, immunoprecipitated with IgG.

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