Fig. 2: YAP O-GlcNAcylation mediates the ferroptosis sensitivity of HCC cells. | Cell Death Discovery

Fig. 2: YAP O-GlcNAcylation mediates the ferroptosis sensitivity of HCC cells.

From: O-GlcNAcylation enhances sensitivity to RSL3-induced ferroptosis via the YAP/TFRC pathway in liver cancer

Fig. 2

A YAP expression levels were determined in control and YAP knockdown Bel-7402 and SMMC-7721 cells treated with PUGNAc. B–D After RSL3 pretreatment, ferroptotic events were evaluated in control and YAP knockdown Bel-7402 and SMMC-7721 cells treated with PUGNAc: cell death (B), lipid ROS production (C), and MDA production (D). E Interaction between O-GlcNAc and YAP using anti-YAP antibody with input as control. F–I Bel-7402 and SMMC-7721 cells were transfected with YAP-sh, and YAP-WT or YAP-T241A was simultaneously overexpressed. Endogenous YAP was immunoprecipitated, and O-GlcNAcylation of YAP was detected by WB (F). After RSL3 and PUGNAc pretreatment, ferroptotic events were evaluated in control and transfected Bel-7402 and SMMC-7721 cells: cell death (G), lipid ROS production (H), and MDA production (I). J, K After RSL3 and PUGNAc pretreatment, the colony formation capacity was measured using a soft agar assay in control cells, and Bel-7402 and SMMC-7721 cells transfected with YAP-sh with simultaneous overexpression of YAP-WT or YAP-T241A.

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