Fig. 3: YAP regulates iron metabolism by targeting TFRC. | Cell Death Discovery

Fig. 3: YAP regulates iron metabolism by targeting TFRC.

From: O-GlcNAcylation enhances sensitivity to RSL3-induced ferroptosis via the YAP/TFRC pathway in liver cancer

Fig. 3

A Results of RNA-seq analysis in control and YAP knockdown SMMC-7721 cells. B The heat map demonstrates gene clustering with three biological replicates. Bright green indicates underexpression, and bright red indicates overexpression. C Ferroptosis-related genes were selected to generate a new integrated heat map according to the RNA-seq data. D and E Transcription levels of TFRC (D) and SLC7A11 (E) were verified by qPCR in SMMC-7721 cells with YAP overexpression or knockdown. F and G Transcription levels of TFRC (F) and SLC7A11 (G) were measured by qPCR in SMMC-7721 cells treated with PUGNAc with or without GlcNAc. H The concentration of iron was determined in Bel-7402 and SMMC-7721 cells with YAP knockdown.

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