Fig. 2: Cul3-SPOP E3 ligase activity is required for SUFU downregulation. | Cell Death Discovery

Fig. 2: Cul3-SPOP E3 ligase activity is required for SUFU downregulation.

From: SPOP–PTEN–SUFU axis promotes progression of clear cell renal cell carcinoma via activating SHH and WNT pathway

Fig. 2

A Schematic diagram shows SPOP interacting with substrates through MATH domain and with Cul3 through BTB/3-box domain. B Western blot analysis of SUFU derived from 293T cells transfected with indicated constructs. Cells were treated with MG132 before harvest. C The experiments in B were performed at least three times, and the abundance of SUFU protein was quantified. D SUFU protein levels were examined by western blot analysis of 293T cells, which were transfected with Flag-SPOP, Flag-MATH, Flag-BTB, or Flag-Δ3-box, respectively. E–G 293T cells were transfected with wild-type SPOP or Y87C and W131G mutants. The changes in SUFU protein expression were measured by western blotting. H The abundance of SUFU protein in E–G was quantified and plotted. I SUFU levels were examined in 293T cells co-expressing SPOP and wild-type CUL3 or Y62G and K711S mutants by western blot analysis. *P < 0.05; **P < 0.01 (unpaired Student’s t test).

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