Fig. 6: SPOP promotes tumor progression via activation of the SUFU-dependent SHH and WNT signaling pathways in ccRCC. | Cell Death Discovery

Fig. 6: SPOP promotes tumor progression via activation of the SUFU-dependent SHH and WNT signaling pathways in ccRCC.

From: SPOP–PTEN–SUFU axis promotes progression of clear cell renal cell carcinoma via activating SHH and WNT pathway

Fig. 6

A GEO analysis of 265 ccRCC samples (GSE73731) showed the correlations among the mRNA expression of SPOP, SUFU, and the target genes of SHH and WNT signaling pathways. B Kaplan–Meier analyses showed the associations between GLI1, PTCH1, β-CATENIN, CD44, and TCF4 expression and survival in patients with ccRCC. C qRT-PCR analysis was performed to evaluate the mRNA levels of SPOP, SUFU, and SHH target genes—GLI1, PTCH1, and WNT target genes—CCND1, CD44, TCF4. D Transcriptional levels of SPOP, SUFU, GLI1, PTCH1, CCND1, CD44, and TCF4 were examined by qRT-PCR. E TOP-flash and FOP-flash reporter activities in controls cells and cells transfected with siRNA were shown. *P < 0.05; **P < 0.01; ***P < 0.001 (unpaired Student’s t test).

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