Fig. 5: Mitf directly interacts with CENP-A in melanocytes. | Cell Death Discovery

Fig. 5: Mitf directly interacts with CENP-A in melanocytes.

From: The protective role of MC1R in chromosome stability and centromeric integrity in melanocytes

Fig. 5

A HA-Mitf and Flag-CENP-A were transduced into HPMs. Binding of Mitf to CEPN-A was confirmed with Co-IP. B Interaction between endogenous Mitf and CEPN-A in HPMS was detected with Co-IP using the indicated antibodies. C GST-Mitf pulled down CENP-A expressed in HEK cells. Blot was probed with antibody against CENP-A. D Whole-cell lysates from HPMs were subjected to the Superdex 200 size-exclusion column to separate proteins at different sizes. The lysates collected at various fractions were separated by SDS–PAGE before immunoblot (IB) analysis.

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