Fig. 2: Nuclear export of BmAtg3 and BmAtg8 is associated with autophagy induction. | Cell Death Discovery

Fig. 2: Nuclear export of BmAtg3 and BmAtg8 is associated with autophagy induction.

From: P300/HDAC1 regulates the acetylation/deacetylation and autophagic activities of LC3/Atg8–PE ubiquitin-like system

Fig. 2

A Protein levels of BmSqstm1 and BmAtg3-c-Myc in BmAtg3-c-Myc-overexpressing BmN cells treated with 1, 2.5, and 5 μM 20E for 6 h or 2, 4, and 8 h starvation. B Protein levels of BmSqstm1 and FLAG-BmAtg8 in FLAG-BmAtg8-overexpressing BmN cells treated with 1, 2.5, and 5 μM 20E for 6 h or 2, 4, and 8 h starvation. C Immunofluorescent staining of BmAtg3-c-Myc and FLAG-BmAtg8 after 1, 2.5, and 5 μM 20E treatments for 6 h in BmN cells. Scale bar: 10 µm. D Immunofluorescent staining of BmAtg3-c-Myc and FLAG-BmAtg8 after 2, 4, or 8 h starvation in BmN cells. Scale bar: 10 µm. E Acetylation levels of BmAtg3 and BmAtg8 after starvation for 4 h. F Transcriptional levels of acetyltransferases BmP300 and deacetylase BmHDAC1 in B. mori fat body from 5L2D to PP2 stage.

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