Fig. 1: Effects of GRP75 on cisplatin-resistance in GC.

A SGC7901 or SGC7901CR cells were treated by different concentrations of cisplatin (0 to 103 μM) for 24 h, respectively. The cell viabilities were determined in triplicate, and the IC50s were calculated. B Prognostic significance of GRP75 [Affy ID: 200691_s_at (HSPA9), http://kmplot.com/analysis/index.php?p=service&cancer=gastric]. C Western blot analysis and qPCR analysis in triplicate of the levels of GRP75 protein (left) and mRNA (right). D and E SGC7901CR cells were transfected by scrambled or GRP75 siRNA, while SGC7901 cells were transfected by scrambled or GRP75 plasmids. Cells were treated with different concentrations of cisplatin for 24 h. The cell viabilities were determined in triplicate, and the IC50s were calculated. Western blot analysis of the GRP75 in GC cells.