Fig. 4: Effects of GRP75 on metabolic reprogramming.
A Western blot (left) and quantitative analysis (right) of the levels of p-AKT, HIF-1α, and c-myc proteins in GC cells. B–F SGC7901CR cells were transfected by scrambled or GRP75 siRNA, while SGC7901 cells were transfected by scrambled or GRP75 plasmids. After then, they were treated with 5 or 2.5 μM of cisplatin for 24 h. B Western blot (left) and quantitative analysis (right) of p-AKT, HIF-1α, and c-myc proteins. C qPCR analysis in triplicate of HK2, PDK1, and LDHA mRNAs. D Glucose uptake ability was evaluated and quantitatively calculated in triplicate. E and F The cell viabilities and cell numbers were determined, and the extent of inhibition was calculated in triplicate.
