Fig. 5: Western blot analysis of changes in expression of apoptosis and signal transduction-related molecules after LEN and IM treatment. | Cell Death Discovery

Fig. 5: Western blot analysis of changes in expression of apoptosis and signal transduction-related molecules after LEN and IM treatment.

From: IMiDs uniquely synergize with TKIs to upregulate apoptosis of Philadelphia chromosome-positive acute lymphoblastic leukemia cells expressing a dominant-negative IKZF1 isoform

Fig. 5

KOPN57bi cells were cultured in the presence or absence of LEN (5 μM) and/or IM (0.5 μM), and were harvested at 24, 48, and 72 h. For western blot analysis, the following antibodies were used; Bim, Bid, Puma, Akt, phospho-Akt, Erk1/2, phospho-Erk1/2, STAT5, and phospho-STAT5 (Cell Signaling Technology, Beverly, MA), Bad, Bcl-xl, Bcl-2, and Bax (BD Bioscience, Franklin Lakes, NJ), Noxa (Abcam plc, Cambridge, UK), and C-myc (Santa Cruz Biotechnology, Dallas, TX). The areas of bands at 72 h were quantified by densitometry and shown as the relative ratio to the control after compensation by α-tubulin expression. A Changes in apoptosis-inhibitory molecules. The blots were stained with specific antibodies against IKZF1, Bcl-2, and Bcl-xL. B Changes in pro-apoptotic molecules. The blots were stained with specific antibodies against Bax, Bim, and Bad. C Changes in pro-apoptotic BH3 only molecules. The blots were stained with specific antibodies against c-Myc, Noxa, Puma, and Bid. D Changes in signal transduction-associated molecules. The blots were stained with antibodies against the whole and phosphorylated forms of signal transduction-associated molecules.

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