Fig. 8: Effect of DEX for inducing apoptosis of KOPN57bi cells.

A Flow cytometric analysis of DEX effect on LEN plus IM-induced apoptosis. KOPN57bi cells were cultured for 48 h in the presence or absence of LEN (5 μM) plus IM (0.5 μM) with or without the addition of 2.5 nM DEX (SIGMA-Aldrich), and flow cytometric analysis was performed after Annexin-V/PI staining. B Comparative analysis of apoptosis-inducing effects between 2-drugs and 3-drugs. KOPN57bi cells were cultured for 48 h in the presence or absence of LEN (5 μM) plus IM (0.5 μM), IM plus DEX (2.5 nM), or LEN plus IM plus DEX, and flow cytometric analysis was performed after Annexin-V/PI staining. The X and Y axes shown in A and B indicate log fluorescence intensities of Annexin-V and PI, respectively. C Effect of DEX on LEN plus IM-induced cytotoxicity. KOPN57bi cells were cultured for 48 h in the presence or absence of LEN (5 μM) plus IM (0.5 μM) with or without the addition of DEX (2.5 nM) and alamarBlue cytotoxic assays were performed. D Dose-dependent effect of DEX on LEN plus IM-induced cytotoxicity. KOPN57bi cells were cultured for 48 h in the presence or absence of LEN (5 μM) plus IM (0.5 μM) with different concentrations (0, 1.25, 2.5, and 5 nM) of DEX, and alamarBlue cytotoxic assays were performed. E Comparative analysis of LEN and POM in 2-drugs and 3-drugs-induced cytotoxicity. KOPN57bi cells were cultured for 48 h in the presence or absence of LEN (5 μM), POM (2.5 μM), or IM (0.5 μM) with or without the addition of DEX (2.5 nM), and alamarBlue cytotoxic assays were performed. F Synergistic effect of LEN, PO, and DEX on cytotoxicity in SU/SR cells harboring T315 I mutation. The Ph+ SU/SR cells with T315I mutation were cultured for 48 h in the presence or absence of PO (5 nM) alone or LEN (5 μM) plus PO with or without the addition of DEX (2.5 nM), and alamarBlue cytotoxic assays were performed.