Fig. 4: Effect of Notch blockade on signaling parameters.
From: DCLK1 isoforms and aberrant Notch signaling in the regulation of human and murine colitis
Representative H&E (A), Alcian blue (B), and Ki-67 (C) staining of uninfected (N), CR-infected (CR), or CR + DBZ-treated (CR + DBZ) Rag1−/− mice (scale bar 100 μm; 7–8 mice/group; n = 3 independent experiments). D Total crypt RNA from the same group of mice was isolated and Notch1 expression was investigated via real-time RT-qPCR. The values were normalized to GAPDH and data presented as fold change. P values = 0.000004 (Notch1), 0.000091 (Hes1); n = 3 independent experiments. E Western blot analysis of NICD in the isolated crypts in the indicated group. β-Actin was used as the loading control. Lower panel. Densitometry showing relative abundance. *,**P < 0.05; n = 3 independent experiments. F Real-time RT-qPCR of Notch downstream target Hes-1 in the isolated crypts from the indicated groups. P values = 0.000091 (Hes1); n = 3 independent experiments. Lower panel showing western blot of Hes-1 and Math-1 in the crypts isolated from the indicated groups. Lamin B was used as the loading control (n = 3 independent experiments).
